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Title: The organisation and development of cranial ectoderm
Author: Henshaw, Liam Andrew
ISNI:       0000 0004 7656 1242
Awarding Body: King's College London
Current Institution: King's College London (University of London)
Date of Award: 2015
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The cranial ectoderm is a prime example of tissue interaction, whereby its constituent cell-types: the placodal primordia, neural crest and neural plate, interact and communicate to produce an intricate, highly specialised system. The deployment of the neural crest and ectodermal placodes is central to the development and evolution of the vertebrate head. The neural crest forms" alongside the neural primordium and migrate widely giving rise to a very broad range of differentiated cell types, while the ectodermal placodes form within the cranial ectoderm and contribute extensively to the sensory apparatus of the head. We have identified a member of the Kelch gene family; Ectoderm Neural Cortex 1 (enc1) that shows dynamic expression in placodes throughout their development along with expression in sub-regions of the neural plate and neural tube. Enc1 is a potential actin binding protein. To explore a possible function of Enc1 in placode formation I have used a morpholino (MO) approach to knock down Enc1 translation. I have also investigated the role of placode induction genes, specifically I have attenuated Eyes-absent (eya1) expression, which has previously been shown to imbue cells with a placodal bias. In addition, there has been very little characterization of early placode and neural plate formation on the cellular scale. As such I aimed to explore the morphology of individual cells in these ectodermal regions throughout development, using Beta-Catenin immunohistochemistry to reveal epithelial organisation.
Supervisor: Bell, Esther Jane de Hauteville ; Clarke, Jonathan David William Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available