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Title: Regulation of flagellar mediated motility in the species Samonella enterica
Author: Albanna, Ayman Mohamed Jaber
ISNI:       0000 0004 7429 9279
Awarding Body: Newcastle University
Current Institution: University of Newcastle upon Tyne
Date of Award: 2017
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Salmonella enterica is considered zoonotic pathogen with capability to colonies on range of plants and animals allowing transmission between them. Whole genome sequence analysis of S. enterica generates a phylogenetic tree comprising of three clades: A1, A2 and B. These 3 clades encompass the known 2,600 serovars used to type S. enterica during clinical outbreaks of salmonellosis. S. enterica exploits the bacterial flagellum to be motile in liquid environments and over surfaces. The genetic regulation of flagellar assembly is an elegant and harmonious system driving assembly of the flagellum from the base upwards. We surveyed the response and changes to flagellar regulation in a cohort of S. enterica serovars. Our analysis encompassed examining phenotypic motility, flagellar gene expression and flagellar abundance depending on nutrient composition. We demonstrated that the timing of flagellar gene expression is consistent across the species but the magnitude of flagellar gene expression varies significantly. The S. enterica flagellar system is bistable, producing a heterogeneous population of motile cells. Our data suggested that population heterogeneity plays a role in the adaptation of S. enterica serovars with respect to motility. The great similarity of the flagellum systems between S.enterica and E.coli gave us a reason to study why flagellar regulation in S.enterica differed from E. coli. Indeed, we replaced the master flagellar regulators, flhDC from E.coli into the S. enterica. We found a significant variation in FlhD4C2 activity through mixing flhD and flhC between both organisms. In conclusion, the diversity and changes we observe in just a small subset of S. enterica serovars and by introducing flhDC homologues has made us reconsider a number of assumptions we make about the regulation of the flagellar system based on model-domesticated strains of S. enterica.
Supervisor: Not available Sponsor: Ministry of Higher Education and Scientific Research in Iraq
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available