Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756431
Title: Radiohalogenation of small biomolecules
Author: Hadi, Usama A. M.
Awarding Body: Loughborough University of Technology
Current Institution: Loughborough University
Date of Award: 1978
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Abstract:
Halogenation of tyrosine, uracil, cytosine, uridine and histidine has been attempted—using both the enzymatic catalysis and chemical oxidation labelling methods—with I-125 and Br-80m. Chemical and enzymatic halogenation gave high yields of iodinated tyrosine, uracil, cytosine, uridine, and histidine, and brominated tyrosine, uracil, and cytosine. Reverse-phase high-pressure liquid chromatography separation and gel filtration were both investigated for use in the isolation and analysis of the labelled products. HPLC was most successful and had the advantages that: (1) the technique was fast enough to use with short-lived radiohalogens, i.e. t1⁄2 ≈ 20mins (such as I-128, t1⁄2 = 25 mins); (2) the products were pure and free from interfering impurities. It was found that the use of chemical oxidation and enzymatic catalysis labelling coupled with reverse phase HPLC separation provided a very rapid method of obtaining high specific activity of radiohalogenated compounds in aqueous solution completely free from buffering agents and non-halogenated parent compounds.
Supervisor: Not available Sponsor: Atomic Energy Commission, Iraq
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.756431  DOI: Not available
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