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Title: Bioavailability and mechanisms of iron uptake from pea ferritin and ferric phosphate nanoparticles using cell culture models
Author: Perfecto, Antonio
ISNI:       0000 0004 7426 9678
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2017
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Iron deficiency is a global health burden. Despite sustained efforts to eradicate iron deficiency, it remains the number one nutritional deficiency in the world, affecting over two billion people [1]. One of the main causes of iron deficiency is insufficient dietary intake and/or low bioavailability. Public health approaches to eradicate iron deficiency include supplementation and food fortification. The aims of my PhD project were to investigate the bioavailability and mechanisms of apical uptake of novel iron forms using cell culture models and thereby provide insight toward improving iron deficiency. Chapter 1 is an introduction to the importance of iron to human health, strategies to alleviate iron deficiency, our current understanding of dietary intestinal iron absorption, and methods for measuring iron bioavailability, with a particular emphasis on the in vitro digestion / Caco-2 cell model. Chapter 2 provides an outline of the basic methods used to study iron uptake in Caco-2 and Hutu-80 cells. Chapter 3 investigates the use of ferric phosphate nanoparticles (NP-FePO4) as a source of iron fortification. NP-FePO4 was kindly donated by Michael Zimmermanï¿1⁄2s group based at ETH Zurich. Chapter 4 focuses on the use of plant-derived ferritin (phytoferritin) as a potential iron supplement. Phytoferritin was extracted and purified from marrowfat peas by Janneke Balkï¿1⁄2s lab group, based at the John Innes Centre. Chapter 5 examines the use of the commercially available iron ingot, Lucky Iron Fish©, as a source of home iron fortification. Chapters 3, 4, and 5 are the basis of 3 separately submitted manuscripts; one of which has been accepted, and the other two currently under peer review. Lastly, a general discussion of the experiments undertaken and recommendations for future work are outlined in Chapter 6. All three iron sources used in this thesis comprise the newest strategies for iron therapeutics. By assessing iron bioavailability, and more importantly the mechanisms of iron uptake, it is hoped that the work will prove insightful and can be used in the design of future prospective human trials.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available