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Title: The interactions of optoelectronically active molecules and complexes with (bio) macromolecules for applications in biosensors and directed assembly
Author: Al-Azzawi, Ammar
ISNI:       0000 0004 7426 6231
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2017
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Since the discovery of DNA as the template for protein synthesis, great effort has been made by researchers to develop DNA as a target for drugs and biosensors. For biosensors small molecules DNA-binder are required that change chemical and physical properties (such as absorbance spectra, redox properties) on binding. Proteins and enzymes are still a predominant target for disease therapy. However, DNA can also be a target for medicine.1 In fact,small molecules which bind to DNA function as drugs or as sensitisers in biosensors. This chapter gives a general overview of the DNA structure. Chapter one starts by introducing some important concepts of development of biosensors. The chapter discusses DNA functions and the mode of interaction of small molecules with doublestranded DNA as well as the types of binding, such as electrostatic, intercalation and groove interactions. Moreover, the chapter briefly describes supramolecular and self-assembled nanostructured materials based on natural and synthetic polymers. The chapter finishes with the biophysical techniques which can be used to quantify the interaction between molecules and duplex DNA, such as UV-visible spectroscopy, circular dichroism (CD) and isothermaltitration calorimetry (ITC).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available