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Title: DNA printing on polymer substrates : towards cost-effective medical diagnostic devices
Author: Ren, Ruobo
Awarding Body: Swansea University
Current Institution: Swansea University
Date of Award: 2011
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DNA for sensing has a diversity o f life science applications. The aim o f this work was to explore the printing DNA onto a flexible substrate. DNA printing has a diversity of life science applications. DNA can be printed onto rigid substrates at high resolution. Printing is a candidate process for volume manufacturing. This required identification o f substrate and any surface treatment to ensure wetting and adhesion. Printing also involves matching the ink with printing process characteristics and for the purpose o f sensing, it requires the formulation o f an ink that can be printed and demonstrates suitable hybridisation characteristics. Each of these aspects was addressed within this thesis. Measurement techniques and their robustness were established as a first step. The next stage focused on determining a suitable substrate and surface treatment for immobilisation o f DNA. This was followed by an exploration o f printing processes, which include inkjet as a reference and flexography and gravure as candidates for volume printing. This work was carried out on a bench top printed, while providing a path for volume printing. The third research strand was concerned with the hybridisation of DNA formulated within an ink. A CCP (Corona treated top coated BOPP) substrate was found to enable good immobilisation o f DNA It needed to be corona treated to enhance the covalent bonding mechanism. Inkjet printing was successfully in applying out, however, within the range o f process parameters explored, neither flexography nor gravure successfully printed DNA. Further work is needed to develop them so they successfully print and could be used for volume production. An appropriate hybridisation method for DNA deposited within an ink formulation was established.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available