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Title: Investigation of the substrate specificity of recombinant Trypanosoma cruzi trans-sialidase
Author: Harrison, Jennifer Amanda
Awarding Body: University of St Andrews
Current Institution: University of St Andrews
Date of Award: 1999
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The protozoan blood-borne parasite Trypanosoma cruzi is the causative agent of Chagas' disease, an enervating and often fatal illness prevalent in South and Central America for which there is no effective treatment. T. cruzi has a cell-surface trans- sialidase which transfers sialic acid from mammalian oligosaccharides to the parasite. This action allows adhesion to and invasion of mammalian cells, subsequently allowing parasitic replication. This protein therefore is exploitable and represents a potential target for the development of chemotherapeutic agents. This thesis describes the purification of recombinant trans-sialidase and the development of a rapid, reliable spectrophotometric coupled assay to measure trans-sialidase activity. It also details the use of three mutually exclusive synthetic oligosaccharide libraries to map substrate recognition for the enzyme. Synthetic fragments of the natural branched oligosaccharide substrates have also been sialylated on a preparative scale, demonstrating the use of trans-sialidase in synthetic oligosaccharide chemistry.
Supervisor: Field, Rob Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QP99.3P7H2 ; Plasma and serum