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Title: Target elucidation of novel trypanosomatid inhibitors
Author: Fraser, Andrew Logan
ISNI:       0000 0004 7234 4005
Awarding Body: University of St Andrews
Current Institution: University of St Andrews
Date of Award: 2018
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In 2010 the Florence group completed the total synthesis of the natural product chamuvarinin which, in collaboration with the Smith group, was found to be a potent inhibitor of the pathogenic parasite T. brucei. Several simplified analogues were found to maintain this inhibitory activity alongside activity against the related species T. cruzi and L. major. The mechanism of action and structural features of these compounds responsible for the observed biological activity remained elusive despite a large synthetic effort by the Florence group. With the aim of identifying protein targets in trypanosomatids to understand the mechanism of action, several photo-affinity labeling analogs have been successfully synthesised and utilised to identify a primary protein target. This protein target was fully validated and molecule docking to this protein was evaluated in-silico. This computational data was used to evaluate the mode of action and aided in the design of simplified compounds which were found to maintain the previously observed anti-parasitic activity but with decreased toxicity to mammalian cells alongside decreased synthetic complexity. The total synthesis of the natural product ascr#18 is also described alongside the synthesis of photo-affinity labeling analogs. This natural product is implicated in the modulation of pathogen resistance in plants and has potential application in crop production.
Supervisor: Florence, Gordon J. Sponsor: Leverhulme Trust ; Engineering and Physical Sciences Research Council (EPSRC)
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: RM411.F8 ; Antiparasitic agents--Design ; Trypanosomatidae ; Natural products