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Title: Generation and characterisation of progenitor cell lines from human fetal kidneys
Author: Mari, Chiara
ISNI:       0000 0004 7232 5664
Awarding Body: UCL (University College London)
Current Institution: University College London (University of London)
Date of Award: 2018
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Kidney disease is a global public health burden. Chronic kidney disease, with an incidence increasing annually in the Western world, is a progressive kidney damage that can lead to end-stage renal failure (CKD5), in which kidney function is completely lost. CKD5 treatment options are dialysis, which is not a cure for kidney disease and kidney transplantation limited by the shortage of donor organs. Kidney disease new interventions have two aims: 1. preventing the progression to CKD5 2. creating a healthy organ in vitro with bioengineered scaffolds in order to permanently replace defective kidneys. The first aim involves the utilisation of drug- and cellular- based therapies to delay or to stop the progression to CKD5. However, this aim is made problematic by the paucity of available primary human renal cell lines to be used for new drugs testing and the incapability to accurately assess the efficacy of cellular-based therapies in appropriate animal models. The PhD Thesis illustrates the attempt in meeting this need. The thesis focuses on the generation and characterisation of human progenitor cell lines and further assessment of their potential to be used as cell-based therapy. I have generated human fetal cell lines from healthy kidneys, based on the expression of known markers of renal progenitor cells, CD24 and CD133. I have characterised their gene expression profile over multiple passages, assessing the expression of progenitor and lineage markers and evaluated their capacity to differentiate towards multiple lineages (osteoblasts, adipocytes and renal epithelia). Furthermore, the potential of these cells to be used as therapy has been assessed in mouse model of acute renal injury induced by folic acid after comparing the course of the folic acid-induced renal injury in immunocompetent and immunodeficient mice strains.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available