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Title: Application of morphometric analysis and tissue engineering to bioengineering personalised autologous bone tissues for the reconstruction of congenital midface deformities
Author: Ibrahim, Amel El-Kabashi Abdullah
ISNI:       0000 0004 7227 991X
Awarding Body: UCL (University College London)
Current Institution: University College London (University of London)
Date of Award: 2017
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Congenital craniofacial bone deformities frequently occur in conditions such as Craniofacial microsomia (CM) and Treacher Collins Syndrome (TCS). Affected children may suffer from functional impairment and poor self-esteem. Reconstruction aims to restore form and function but often involves multiple invasive surgeries throughout childhood. The reliance on foreign-body implants and autologous tissue-transfer is also associated with morbidity. The aim of this work was to assess whether morphometric analysis and tissue engineering using paediatric adipose derived stem cells could facilitate bioengineering personalised autologous facial bone implants to provide a more accurate and life-long solution for the treatment of midface deformities. Paediatric facial CT scans (n=70) from control, CM and TCS subjects were used to build a dense surface model of the midface to study normal and dysmorphic postnatal midface development. This enabled relating of soft and skeletal tissue growth, analysis of asymmetry and evaluation of surgical correction. This work also establishes the foundations for developing a surgical planning tool. Paediatric craniofacial bone was also analysed in order to establish a reference for tissue engineering and reverse engineer the bone microenvironment to fabricate biomaterials and culture conditions that enhance osteogenic maturation. It was possible to bioengineer bone tissue using hADSC cultured on a bone biomimetic hybrid POSS-PCL-Fibrin scaffold. Cellularised scaffolds survived subcutaneous implantation in nude mice for 4 months, underwent vascularisation and showed evidence of mature extracellular matrix formation and cellular composition similar to native bone The results of this work support a multi-faceted approach to bone tissue engineering. Increased understanding of paediatric facial bones permits recreation of the bone microenvironment to enable osteogenic maturation of hADSC. These tissues could eventually be custom-shaped using an operative planning tool based on these computer models. Future work using larger data sets, bioreactors, 3D printing and large animal defect models will seek to build on these promising results.
Supervisor: Ferretti, P. ; Hammond, P. ; Bulstrode, N. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available