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Title: Development of resorbable nanocomposite polymers for paediatric airway tissue engineering
Author: Teoh, G. Z.
ISNI:       0000 0004 7229 9793
Awarding Body: UCL (University College London)
Current Institution: University College London (University of London)
Date of Award: 2016
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Advances in stem cell technology, tissue engineering and material sciences have inspired the development of resorbable, nanocomposite polymers for airway tissue engineering. At present, tracheal resection with primary repair is the Gold Standard of care for those with airway disorders; however, the maximum resectable length of the trachea is restricted to 30% of the total length in children. Replacement of longer sections thus requires the development of a safe, functional tracheal prosthesis as there are currently few clinically available options and attempts to provide autologous grafts for human application have been disappointing for a host of different reasons, including lack of integration and poor mechanical strength. Three new ethylene-diethylene glycol based nanocomposite polymers were synthesized and characterized from material and biological perspectives. Material characterization studies included polymer viscosity, tensile strength, suture retention and thromboelastography, in addition to an examination of the degradation profiles. Laminated bilayer polymeric scaffolds were then seeded with human tracheobronchial epithelial cells (HBECs) and bone marrow derived mesenchymal stem cells (bmMSCs) for an in vitro assessment of cytocompatibility before proceeding to in vivo studies examining scaffold integration and vascularization as well as whole construct behavior following orthotropic implantation. In vitro and in vivo results were positive, with key findings including good cell attachment and proliferation, formation of vasculature and a suitable in vivo degradation profile. Overall, a promising material candidate for use in airway tissue engineering has been developed. However, further optimization and standardization of the small diameter tracheal constructs manufactured using POSS-enhanced ethylene-diethylene glycol based polymers is required prior to embarking on pre-clinical and clinical trials exploring the use of the scaffolds for treatment of tracheal anomalies and pathologies in clinics.
Supervisor: Cousins, B. G. ; Birchall, M. A. ; Seifalian, A. M. ; Song, W. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available