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Title: ARGONAUTE10 inhibits INDEHISCENT to regulate shoot apical meristem and replum development in Arabidopsis thaliana
Author: Valluru, Manoj Kumar
ISNI:       0000 0004 7226 315X
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2017
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ARGONAUTE10 (AGO10) regulates shoot apical meristem (SAM) and gynoecium development by controlling Class III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) transcription factor expression. The ago10zwl-3 mutant failed to establish SAM, leaf adaxial identity, and carpel margin meristems (CMMs). However, the factors that act downstream of AGO10 and HD-ZIP III genes to regulate development are not known. This work has identified that the INDEHISCENT (IND) bHLH transcription factor functions downstream of AGO10 and HD-ZIP III genes to ensure proper SAM and replum development. IND overexpression causes SAM defects similar to ago10zwl-3 mutants, and the ind mutation partially rescues ago10 mutant phenotypes. IND overexpression negatively regulates tissue bilateral symmetry by repressing polar auxin transport (PAT), AGO10 and probably CUC1 expression. However, HD-ZIP III transcription factors PHB and REV indirectly repress IND and promote CUC1 expression. AGO10 and IND regulate each other antagonistically. AGO10 repression of IND is essential for SAM and replum development because overexpression of IND impairs tissue bilateral symmetry. This is the first study to demonstrate a role for IND in SAM development and that the main function of AGO10 is to maintain proper IND expression.
Supervisor: Sorefan, Karim Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available