Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.743490
Title: A study of the histones of Euglena gracilis and other protozoa
Author: Jardine, Nicholas Jeremy
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1975
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
A method has been developed for the isolation of nuclei from the flagellated protozoan Cuglena gracilis, and histone Isolated from the nuclei has been extensively studied. The amino acid composition of the unfractionated Euglena histone is similar to that for the histone of higher organisms. Euglena histone was separated by gel filtration into five fractions and the individual fractions were further purified by selective precipitation and solubility techniques. Amino acid analysis of the purified proteins indicated the presence of f1, f2b, f2al and f3 histones. The remaining fraction, designated f2a2, differed from vertebrate f2a2 in having a higher lysinet arginino ratio. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate indicated that the sizes of the individual Euglena histones are similar to the homologous vertebrate fractions. Using electrophoretic techniques, a comparison was made of Euglena histone and histones isolated from rat liver, trypanosomatid organisms and the colourless euglenold Astasia longa. The nature and complexity of the histones from the various organisms were broadly similar, but some differences in the electrophoretic patterns were evident.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.743490  DOI: Not available
Share: