Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.742912
Title: Development of analytical methods for the stability assessment of parenteral nutrition
Author: Emery, Sophie
ISNI:       0000 0004 7224 2682
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2018
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Abstract:
Parenteral nutrition (PN) provides intravenous nutritional support to patients with reduced gastrointestinal function. A PN bag comprises the basic building blocks of the food groups: lipids, glucose, amino acids, vitamins, electrolytes and trace elements. Recently there has been an increase in demand for extended storage periods for PN bags, to ease management of an increasing home care market. Prior to a PN formulation being deemed safe for a patient, a laboratory simulation is carried out on the proposed admixture under the requested storage and administration conditions. Currently only the physical stability is assessed; physical testing provides no information on the quantity of each component remaining in the bag after storage. Consequently, there is a need for assessing the chemical stability of PN to indicate the quantity of each component that remains in the PN bag. A commonly used amino acid product, Aminoven® 25, contains 16 amino acids; this work aimed to develop a HPLC assay capable of quantifying the amino acids in an aqueous PN bag containing Aminoven® 25. Fluorescence detection was used as it is a highly selective method of detection, which was preferable due to the number of components in PN. To detect the amino acids, as they don’t naturally fluoresce, derivatization was carried out using ortho-phthalaldehyde to form a fluorescing derivative. The developed assay resulted in validation of thirteen of the amino acids in Aminoven® 25. In addition, the method was shown to be unaffected by the iv presence of aqueous PN components, so this method is suitable for quantifying thirteen amino acids in aqueous PN containing Aminoven® 25. This assay can be used for assessing the stability during stability testing and confirming the quantity of amino acids after compounding for quality control release.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.742912  DOI: Not available
Keywords: R Medicine (General) ; RM Therapeutics. Pharmacology
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