Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.739687
Title: Electromagnetic sensing of cell suspensions in microfluidic systems
Author: Watts, Clare
ISNI:       0000 0004 7229 4159
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2018
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Abstract:
Cancer is one of the leading causes of death worldwide, and cancers which metastasise, the process of cancer spreading from one organ to another within the body, have the highest fatality rates. Although the mechanism of metastasis is not well understood, the study of cells in the circulatory system which are known to seed tumours is of great importance in cancer research. Current methods for detection of such cells, although specific and sensitive, result in their destruction, and have drawbacks associated with a bias towards specific cell markers. This work seeks to assess the feasibility of using dielectric spectroscopy and the variation in dielectric properties of different cells at microwave frequencies to characterise and separate circulating tumour cells (CTCs) and other abnormal cells from peripheral circulating blood. This technique is non-destructive and label free, and opens the door to a flow-cytometer system based purely on the physical properties of different cells, capable of measuring them in their natural state. A novel coupled-resonator sensor is introduced in this work, and used to make dielectric measurements of polystyrene microspheres as well as single, flowing, viable cells of three different types at ~10 GHz. The results have been compared to Raman spectroscopy analysis of the cultured cells, showing potential correlation between the water content of the cell types investigated, and the response of the novel system. This is of diagnostic value, with cell hydration linked to carcinogenesis.
Supervisor: Klein, Norbert ; Stingelin, Natalie Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.739687  DOI:
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