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Title: Serum albumin measurement in some species of domestic animals : a dye-binding and electrophoretic technique compared
Author: Keay, George
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1981
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This work is centred on the measurement of serum proteins and in particular, albumin, in three species of domestic animals, bovine, ovine and equine. The main methods of protein estimation are reviewed on a historical basis and the various modifications and improvements to each are discussed. During the course of the discussion emphasis is given to the development of methods of protein fractionation by electrophoresis, by the binding of various indicator dyes to albumin and to the results obtained with animal sera. Although investigated recently in the medical field, very little attention has been paid in the veterinary field to the observation that bromocresol green (B.C.G.) dye is not bound entirely specifically by albumin and that it also undergoes an additional, time dependent reaction with certain other serum globulins. Comparisons between human serum albumin results by this dye-binding method and more specific techniques have shown that albumin levels by the 'immediate' (< .30 secs.) reaction wdth B.C.G. dye are the most accurate. Only one group of veterinary research workers have employed this reaction time when investigating albumin levels in animal serum. The degrees of difference between albumin levels obtained using the 'immediate' and the originally described 10 minute B.C.G. reactions however, have not been demonstrated and part of this work is devoted to the identification of such differences. The effect of using non-specific albumin standards and the reaction of specific purified globulin or albuminfree serum with B.C.G. dye is also investigated. The remainder of this work is devoted to various aspects f the electrophoretic behaviour of serum proteins and the ecognition of the fractions which are clearly identifiable after garose gel electrophoresis. The main findings of this work were:- (a) There was a positive difference in albumin levels between those obtained by the 'immediate' and by the 10 minute B.C.G. reactions in the three species. The widest of -these differences was observed when bovine serum was analysed. (b) Only albumin values obtained by the 'immediate' B.C.G. reaction were in good agreement with a more specific method of albumin measurement. (c) Electrophoretic albumin values were also in good agreement with those by the 'immediate' B.C.G. reaction. (d) Equivalent amounts of purified albumin of each species did not react identically with the dye and the use of albumin standards in the B.C.G. method which were not species specific led to statistically significant discrepancies in albumin results. (e) Purified gamma globulin of each species did not react with B.C.G. dye, but other globulins did, although unlike albumin, not on a weight for weight basis. The reactivities of equivalent amounts of alpha and beta globulins of each species appeared not to be identical. (f) A set of normal serum protein values was determined for each species using the main methods described in this study.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available