Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.731568
Title: Phosphoinositides in ciliary transport and ciliopathies
Author: Fang, Xiaoming
ISNI:       0000 0004 6497 7347
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2018
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Abstract:
Cilia are finger-like protrusions that function in the detection and processing of a wide variety of signals. In vertebrates, they are intimately involved in early embryonic patterning, the differentiation and function of sensory neurons, morphogenesis and physiology of duct epithelia, cell motility and metabolism. Lipid composition of the ciliary membrane and its contribution to cilia function is one of the least studied areas of cilia biology. Here I use a collection of transgenic lines to determine the phosphoinositide content of the ciliary membrane and to test the role of phosphoinositides in cilia morphogenesis and function. My studies reveal that specific types of phosphoinositides are found in the ciliary membrane and that their content plays an important role in cilia morphology and function. Moreover, I generated a zebrafish model of a human ciliopathy to test whether some aspects of its mutant phenotype can be alleviated by manipulating the phosphoinositide content in the ciliary membrane. Finally, my studies developed a method that is generally applicable to the manipulation of ciliary content of other PIs in a living vertebrate using other enzymes.
Supervisor: Malicki, Jarema Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.731568  DOI: Not available
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