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Title: Imaging lipid phase separation in droplet interface bilayers
Author: Danial, John Shokri Hanna
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 2015
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The spatiotemporal organization of membrane proteins is implicated in cellular trafficking, signalling and reception. It was proposed that biological membranes partition into lipid rafts that can promote and control the organization of membrane proteins to localize the mentioned processes. Lipid rafts are thought to be transient (microseconds) and small (nanometers), rendering their detection a challenging task. To circumvent this problem, multi-component artificial membrane systems are deployed to study the segregation of lipids at longer time and length scales. In this thesis, multi-component Droplet Interface Bilayers (DIBs) were imaged using fluorescence and interferometric scattering microscopy. DIBs were used to examine and manipulate microscopic lipid domains and to observe, for the first time, transient nanoscopic lipid domains. The techniques and results described here will have important implications on future research in this field.
Supervisor: Wallace, Mark Sponsor: Weidenfeld Scholarships and Leadership Programme
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Biophysical Chemistry ; Biophysics ; Biophotonics ; Membrane Chemistry