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Title: Bioactive peptides from the defensive skin secretions of selected American and European frogs : a comparative study
Author: Liu, Siqin
ISNI:       0000 0004 6424 6560
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2017
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An antimicrobial peptide (AMP) belonging to the brevinin-2 family was isolated and characterised from the skin secretion of the European edible frog, Pelophylax. kl. esculentus. Skin secretions, as the source of mRNA, were harvested from the dorsal granular glands and a cDNA library was constructed by using a BD SMART II synthesis kit. The open-reading frame of a cloned cDNA transcript was translated and analysed through bio-informatics which revealed that it encoded the precursor of an AMP which was named brevinin-2Ta (B-2Ta). B-2Ta exhibited remarkable antimicrobial and anticancer cell activities. The in vitro assays showed that synthetic B-2Ta inhibited S. aureus, E. coli and C. albicans with MIC values of 64mg/L, 32mg/L and 64mg/L, respectively. In addition, the peptide also possessed obvious inhibitory activities against the cancer cell lines, PC-3, H23, and MDA-MB-435s. The cytotoxicity study revealed a low degree (3%-9%) of haemolysis at MIC concentrations. In conclusion, this amphibian skin AMP has possible therapeutic potential as an anti-infective or anti-cancer agent and may have the virtue of preventing multi-drug resistance.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available