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Title: The expression and function of airway epithelial Transient Receptor Potential (TRP) channels in hypersensitive airways in children with respiratory problems
Author: Anandarajan, Mugilan
ISNI:       0000 0004 6423 229X
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2017
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Aim' To determine whether the bronchial epithelial cells from young children with different wheezing or coughing phenotypes have increased expression of transient receptor potential (TRP) cation channel receptors compared to normal. Hypotheses to be tested: TRPV1, TRPV4, TRPA1 and TRPM8 receptors are expressed and over-expressed on the bronchial epithelium of young children with post bronchiolitis wheezing / episodic viral induced wheezing (non- asthmatic wheezers), classical atopy asthma and coughers compared to normal subjects. Methodology: Freshly isolated bronchial epithelial cells from children were tested for expression of TRP channels and their functional role by microfluorimetry, immunocytochemistry, confocal live cell imaging, patch clamping and qt- PCR. Results: The experiments conducted in the present study has aided in inferring that TRPV1, V4, A1 and M8 channels are expressed and are functional in bronchial epithelial cells. The channels are expressed in sub cellular organelles in the peri nuclear and nuclear region and are involved in calcium signaling. Our data from microfluorimetry and confocal live cell experiments strongly suggests that TRPM8, V1, V4 and A1 channels are functionally active as evidenced by their ability to 2+ contribute to PBEC Ca signaling and there were differences in response of freshly isolated bronchial epithelial cells between asthmatic and healthy children. However further tests needed to be carried out with more patient samples to analyze the significance of the differences. The analysis of responses from confocal live cell imaging suggested that the calcium whole cell responses and the peak responses in microfluorimetry experiments were increased in most asthmatic cells in comparison to Freshly isolated bronchial epithelial cells from healthy children. Despite the number of patient samples and cells analyzed this is a significant finding suggesting possible up regulation or over expression of the TRP channels in children with asthma. The present study has shown the presence of TRPV1, V4, A1 and M8 channels in bronchial epithelium with possible increased expression or upregulation of these channels in asthma compared to normal healthy children.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available