Use this URL to cite or link to this record in EThOS:
Title: Development and integration of chemical imaging methods for applications in biomedical and pharmaceutical research
Author: Randall, Elizabeth Claire
ISNI:       0000 0004 6349 9005
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2017
Availability of Full Text:
Access from EThOS:
Access from Institution:
Imaging of biomolecules in biological substrates by mass spectrometry or spectroscopic imaging techniques plays a major role in biomedical, clinical, and pharmaceutical research. The work presented in this thesis investigates the capabilities of three imaging techniques, liquid extraction surface analysis (LESA) mass spectrometry imaging (MSI), matrix assisted laser desorption ionisation (MALDI) MSI and stimulated Raman scattering (SRS) microscopy. A method for combined LESA and MALDI analysis was developed and results provided high resolution imaging of multiple analyte classes (proteins, lipids and small molecule drugs) in thin tissue sections. SRS microscopy was used for the quantitative imaging of MALDI sampling effects and sample preparation, providing insight into fundamental processes of MALDI MS. Multimodal SRS, LESA and MALDI imaging was executed on a single tissue sample revealing the complementarity between the three approaches. Specific challenges for LESA were further explored, namely quantification, improved spatial resolution and alternative biological substrates. A quantitative LESA method based on the production of mimetic tissue models containing stable isotope-labelled proteins was developed. An alternative platform, the Flow-Probe™, with the potential to achieve higher spatial resolution was assessed. Finally, a LESA method for the direct analysis of proteins from live bacterial colonies was developed.
Supervisor: Not available Sponsor: Engineering and Physical Sciences Research Council (EPSRC) ; National Physical Laboratory
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QD Chemistry