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Title: Advanced light-sheet and structured illumination microscopy techniques for neuroscience and disease diagnosis
Author: Nylk, Jonathan
ISNI:       0000 0004 6347 924X
Awarding Body: University of St Andrews
Current Institution: University of St Andrews
Date of Award: 2016
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Optical microscopy is a cornerstone of biomedical research. Advances in optical techniques enable specific, high resolution, sterile, and biologically compatible imaging. In particular, beam shaping has been used to tailor microscopy techniques to enhance microscope performance. The aim of this Thesis is to investigate the use of novel beam shaping techniques in emerging optical microscopy methods, and to apply these methods in biomedicine. To overcome the challenges associated with high resolution imaging of large specimens, the use of Airy beams and related techniques are applied to light-sheet microscopy. This approach increases the field-of-view that can be imaged at high resolution by over an order of magnitude compared to standard Gaussian beam based light-sheet microscopy, has reduced phototoxicity, and can be implemented with a low-cost optical system. Advanced implementations show promise for imaging at depth within turbid tissue, in particular for neuroscience. Super-resolution microscopy techniques enhance the spatial resolution of optical methods. Structured illumination microscopy is investigated as an alternative for electron microscopy in disease diagnosis, capable of visualising pathologically relevant features of kidney disease. Separately, compact optical manipulation methods are developed with the aim of adding functionality to super-resolution techniques.
Supervisor: Dholakia, Kishan Sponsor: Engineering and Physical Sciences Research Council (EPSRC)
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Microscopy ; Beam shaping ; Wavefront shaping ; Fluorescence ; Light-sheet microscopy ; LSM ; Structured illumination microscopy ; SIM ; Optical manipulation ; Optical trapping ; Airy beam ; Bessel beam ; Gaussiam beam ; Physics ; QH207.N8