Use this URL to cite or link to this record in EThOS: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.719940 |
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Title: | Investigating the in vivo functions of non-coding RNAs in Drosophila melanogaster | ||||||
Author: | Schwarz, Jenna |
ISNI:
0000 0004 6346 5171
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Awarding Body: | University of Oxford | ||||||
Current Institution: | University of Oxford | ||||||
Date of Award: | 2015 | ||||||
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Abstract: | |||||||
The number of annotated non-protein coding genes has increased dramatically in recent years, but the functions of very few are well understood. In this thesis, I study the functions of two classes of non-coding RNA in Drosophila melanogaster. Firstly, through characterisation of six putative long non-coding RNA loci containing transposon insertions, I identified a long non-coding RNA, lnc703, suitable for in depth characterisation. Using a GAL4/UAS fluorescent reporter I found lnc703 to be expressed in the larval fat body and confirmed this using qRT-PCR. Transcriptome profiling identified ribosomal genes as upregulated in two independent lnc703 mutant strains. Additionally, I have identified decreased lifespan in some lnc703gal4 strains and it will be interesting to determine whether there is a relationship between increased ribosomal protein gene expression and reduced lifespan. I also used transcriptome profiling of the musculature and CNS of miR-8 null larvae to determine tissue specific targets of miR-8. Many indirect effects of miR-8 were detected but using MRE prediction algorithms a number of potentially direct targets of miR-8 were identified. One of these, ilp5, which is upregulated in the CNS, was identified as a non-cell autonomous effect of loss of miR-8 in the fat body. Together this work highlights the importance of using multiple, complementary techniques to study the function of non-coding RNAs in vivo.
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Supervisor: | Liu, Jilong ; Ponting, Christopher | Sponsor: | Medical Research Council ; European Research Council | ||||
Qualification Name: | Thesis (Ph.D.) | Qualification Level: | Doctoral | ||||
EThOS ID: | uk.bl.ethos.719940 | DOI: | Not available | ||||
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