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Title: The role of anti-Mullerian hormone in the ovary : relevance to polycystic ovary syndrome
Author: Dilaver, Nafi Mehmet
ISNI:       0000 0004 6349 7819
Awarding Body: St George's, University of London
Current Institution: St George's, University of London
Date of Award: 2017
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Anti-Mullerian hormone (AMH) is secreted by granulosa cells (GC) in the ovary and is produced in much higher amounts by GCs in women with polycystic ovary syndrome (PCOS). The aim of this project was to examine the role of AMH in the normal ovary and thereby understand the consequences for follicle development of greater concentrations found in women with PCOS. Human granulosa-luteal cells (GLCs), human theca tissue and KGN cells were used to investigate the effect of AMH on GC growth and apoptosis. Furthermore, the relationship between AMH and the essential gonadotrophins of folliculogenesis, i.e. FSH and LH were investigated. AMH signalling was also investigated in GLCs from women with and without PCOS. In addition, the effect of AMH on whole genome expression in GLCs from women with normal ovaries and PCOS was determined. AMH inhibited GC proliferation but did not appear to do this via apoptosis. AMH also had a differential effect on SMAD proteins in normal and PCOS cells. Interestingly, AMH increased inhibitory SMADs 6 & 7 and decreased pSMAD 1/5/8 in PCOS cells compared to basal levels. Finally, AMH had a significant differential effect on the transcriptome profile of cells from women with normal ovaries and PCOS. In summary, a differential effect of AMH in cells from women with normal ovaries and PCOS has been demonstrated, with evidence to speculate that the inhibitory role of AMH in the ovary may be due to an effect on the cell cycle.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available