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Title: Heterologous expression and characterisation of [beta]-xylosidases from fungal sources
Author: Kirikyali, Narin
ISNI:       0000 0004 6352 9577
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2014
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Beta-xylosidases (E.C. are a subclass of glycoside hydrolases (E C. 3.2.1.-) which hydrolyses the pi,4 glycosidic linkages of short chain xylo- oligosaccharides and xylobiose from the non-reducing termini releasing xylose monomers. They constitute a group of enzymes that have the biotechnological potential use in various industrial processes. As a consequence it has been a necessity for enzymes involved in hemicellulose hydrolysis, of industrial interest, to be individually identified and characterised in order to utilise them for practical purposes. In order to expand the range of available Beta-xylosidases, we have examined and characterised novel p-xvlosidases from Neurospora crassa, Aspergillus oryzae and Humicola insolens. To provide a supply of Beta-xvlosidases, the encoding genes from Neurospora crassa and Aspergillus oryzae have been cloned and over-expressed in the Pichia pastoris expression system. The kinetic behaviour of these two enzymes is discussed in terms of their roles in the presence of synthetic and natural substrates and inhibitors.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available