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Title: Functional genomic studies on skin secretions of frogs from the genus Kassina
Author: Zhang, Huiling
ISNI:       0000 0004 6060 245X
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2016
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In this thesis, skin secretions from member species of the African frog genus, Kassina, (Kassina maculata and Kassina senegalensis) were used as a source of bioactive peptides. By employment of molecular cloning technology, four bioactive peptides were identified and characterised from electrically-stimulated skin secretions of both species and the bioassays described in this thesis were all performed using synthetic replicates of each peptide. Kassina senegalensis skin secretion is considered as one of the richest sources of tachykinins. In this thesis, two tachykinin-related peptides are described. Chapter 3 describes a peptide which is structurally and functionally similar to kassinin, which was found to stimulate the activity of isolated urinary bladder and uterus smooth muscle preparations in a dose-dependent manner. A second peptide described in Chapter 4, proved to be a tachykinin antagonist and specifically inhibited the contraction effect of kassinin and neurokinin A (NKA) on rat urinary bladder. This is a natural non-competitive NK-2 receptor antagonist and the inhibitory effect could was comparable to an established synthetic NK-2 receptor antagonist. Due to its inhibition of kassinin effects and its position behind kassinin on their common precursor, this peptide was named kasshinder.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available