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Title: Diversity, stress responses and ecological behaviour of Mycobacterium species
Author: Santos, Ricardo
ISNI:       0000 0004 6060 2265
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2016
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Mycobacterium is one of the oldest known bacterial genera and its species are commonly associated with human and animal disease, although most are free-living saprophytes which form part of a balanced microbial community in natural habitats such as water and soil Mycobacteria are highly distributed in the environment and in man-made water infrastructures mainly due to its robustness and ability to adapt Until now there are only scarce reports of their presence in extreme environments but no methodic and large-scale survey of these environments has been done in order to truly understand their distribution in such environments. This thesis encompasses the isolation of strains from 263 different positive samples and their identification using different techniques. The most commonly isolated species were Mycobacterium gordonae in Glacier National Park and Mycobacterium parascrofulaceum and Mycobacterium avium in Yellowstone National Park. Isolates were tested for their resistance co temperature, pH and different stressors, in an attempt to explain the remarkable resistance for most of the tested conditions, several cell characteristics were tested, including fatty acids composition, polyphosphate accumulation and catalase activity. Scanning Electron Microscopy was performed to have an insight into the genera! structure of the cells. Results emphasize the highly resilience of mycobacteria to different types of environmental stress. From the tested conditions, the subtle differences In the fatty add composition, in the catalase activity and polyphosphate accumulation, demonstrates that the formation of biofilm-like structures helps to understand cite highly resistance of the members of this genus.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available