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Title: Functional genomic studies on the skin secretions of selected South American frogs
Author: Yang, Nan
ISNI:       0000 0004 6060 1801
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2016
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Amphibians, as their name implies, are creatures that can live in two different kinds of ecological environments -land and water, and they occur with a wide global range except for Antarctica. It is amazing that amphibians have had such a long history, with seemingly fragile, naked skin, without scales like reptiles, feathers like birds, or hair like mammals, not to mention their lack of sharp teeth and claws. Actually, their skin secretions have kept them alive and protected from infections by microorganisms and from attacks of predators due to the complex arrays of chemicals which they contain. In this thesis, eight skin-derived peptides were isolated by molecular cloning, chemically-synthesised and biologically-evaluated and results are described in detail in Chapters 3 through 6. In Chapter 3, two novel phylloseptins are described together with their similar sequences and antimicrobial potential. In Chapter 4, a mature BRP with three structural variants, were tested and compared, in order to assess the effects of hydroxylation and post-translational modification. In Chapter 5, a novel peptide, G9I, was isolated from Phyllomedusa tarsius and functionally screened. In Chapter 6, dermaseptin-3 was successfully isolated from Phyllomedusa tarsius. The peptide exhibited potent antimicrobial activity against all the three types of microorganism tested, as well as having an anti-cancer property.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available