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Title: Studies in paternal genomics and proteomics and their impact on male reproductive potential
Author: Kumar, Kishlay
ISNI:       0000 0004 6057 0822
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2016
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The primary aim of this thesis was to examine major aspects of paternal genomics (by sperm DNA damage) and sperm protein expression (by proteomics) and their possible impact on male reproductive potential. In order to achieve this aim a number of studies were undertaken. To date, no relationships have been reported between ICSI outcomes and sperm DNA quality. Here, a novel assay specific to double stranded DNA damage was employed to see if a relationship was present: 1. The relationship between intracytoplasmic sperm injection (ICSI) outcomes and sperm DNA : fragmentation (single and double stranded DNA breaks) by alkaline and neutral Comet assays. A further attempt to find a biomarker for ICSI failure was performed using the sperm proteome associated with sperm DNA damage: 2. A study of seminal parameters and sperm proteome and their association with sperm nuclear DNA fragmentation following ICSI treatment. To identify areas of vulnerability within the male reproductive tract, a knock out animal model was employed to characterise DNA damage throughout the epididymis: 3. The characterisation of sperm DNA quality in HR6B (Ubiquitin-conjugating DNA repair enzyme) male knockout mice It has been reported that damage to the sperm genome and proteome can occur during routine lab procedures: 4. Here the effects of cryopreservation on the sperm proteome were assessed using high-throughput differential proteomics. The application of the well studied sperm DNA assays; alkaline Comet and TUNEL were compared with the novel neutral Comet in two disease models: a male reproductive and non reproductive cancer: 5. The characterization of sperm DNA quality in men presenting with Cancer.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available