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Title: Examining the anti-diabetic potential of dairy proteins : effects on enteroendocrine cells
Author: Gillespie, Anna Louise
ISNI:       0000 0004 6058 356X
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2016
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A growing body of evidence depicts an inverse relationship between the consumption of dairy foods and the development of metabolic syndrome and/or type 2 diabetes (T2DM). Harnessing the bioactive properties of dairy to aid self-management of T2DM and to enhance the effectiveness of current T2DM treatments remains an attractive and realistic prospect. The studies presented in this thesis investigated i) the potential of using locally sourced yogurt whey as a source of beta-lactoglobulin, ii) the bioactivity of whey and casein proteins to acutely stimulate the biosynthesis and release of the incretin hormones and iii) the usefulness of the pGIP/Neo cell line (a sub-clone of the established STC-1 cell line) for in vitro studies assessing bioactivity. The potential of using yogurt whey as a source of beta-lactoglobulin was not realised in this thesis due to issues such as co-precipitation of an undesirable protein. However, when investigating the GLP-1 secretagogue potential whey proteins, interesting and potentially beneficial results were reported. Overall it appears intact proteins have stimulatory effects on GLP-1 biosynthesis and secretion, however this appears to diminish upon hydrolysis with digestive enzymes. Interestingly, a similar phenomenon was reported for casein and individual casein proteins. Furthermore, dairy proteins have a proliferative effect on EE cells, an attribute that could be useful in the real-life setting. Importantly dairy proteins do not appear to affect the viability and health of EE cells. This thesis also further characterises the pGIP/Neo cell line and reports its potential as a cell line model for GIP secretion. We report for the first time that GIP may be translationally regulated more so than transcriptionally. The work conducted in this PhD thesis further advances the knowledge and understanding of the bioactivity of whey and casein proteins in relation to the incretin hormones and their effect on enteroendocrine cells.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available