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Title: Rational design, assembly and characterization of G-Wires
Author: O'Kane, Christopher E.
ISNI:       0000 0004 6057 4997
Awarding Body: Ulster University
Current Institution: Ulster University
Date of Award: 2016
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G-Wires are supramolecular DNA structures composing of a quadruplex stem. These structures have been reported to have potential for photo and electrical conductance. However, this has yet to be realized. This thesis is concerned with the rational design of DNA sequences in order to form well-structured G-Wires through optimized self-assembly and characterization of the products. Previous investigation has demonstrated that the quadruplex topology formed from G-rich DNA can be controlled through geometric constraints. These constraints can be imposed through various sequence arrangements which lead to specific structural features. A set of systematically varied DNA sequences were designed to form G-Wire structures. These sequences underwent self-assembly in a range of assembly conditions and the self-assembly products were characterized by a range of techniques such as gel-electrophoresis, NMR, UV-spectroscopy and fluorescence spectroscopy. The findings of this investigation have shown that the rational design of DNA sequences successfully resulted in the formation of architecture with unique optical properties and significantly increased quantum yields. This work has the potential to further the ability of G-Wire structure to be utilized within multicomplex nanodevices in applications such as diagnostic sensing and nanoelectronics.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available