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Title: Analysis of a candidate gene for the control of floral heteromorphy in Primula vulgaris
Author: Kent, Olivia
ISNI:       0000 0004 6058 7923
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2016
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Primula vulgaris is a model species for the study of heterostyly, and displays two floral morphologies, pin and thrum, which show a reciprocal arrangement of the anthers and stigma. The differences in floral morphology are controlled by the S locus, which consists of several closely linked genes. One of the first genes to be identified as part of the S locus was GLOT, a paralogue of GLOBOSA. Preliminary results have shown that GLOT expression is confined to the second and third floral whorls of thrum flowers. In pin flowers, where GLOT is not expressed, the anthers are lowered. This study involves characterisation of the expression dynamics of GLOT in comparison to its paralogue GLO, in the context of the recent discovery that the S locus is hemizygous and not heterozygous as previously thought. The selection of normalisation genes for qPCR was conducted, and the temporal expression of both GLO and GLOT was measured across bud development; the genes showed different expression patterns. RNA in situ hybridisation was then used to assess spatial expression of both genes in floral meristems, with GLO showing defined localisation within the developing second and third whorls and GLOT showing more dispersed expression. The interactions of P. vulgaris GLO and GLOT proteins with A. thaliana MADS box proteins were tested in Yeast 2-Hybrid experiments, and while GLO showed interactions with the orthologue of its partner, AP3, GLOT did not show interactions with any of the other proteins tested. Antibodies were designed against peptide sequences to assess protein localisation for use in future experiments. This work has furthered knowledge on the expression patterns of these genes, the divergence of GLOT from GLO, and has generated tools that will enable further analyses of the differences between these two genes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available