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Title: Studies in the turnover of the bound phosphate of skeletal muscle during contraction and relaxation
Author: Whitehead, Susan Elizabeth Aannesley
Awarding Body: University of London
Current Institution: Royal Holloway, University of London
Date of Award: 1970
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The possibility that there is a turnover in active muscle of a phosphate fraction which remains attached to the muscle after extraction with aqueous media, was investigated further. A reversible decrease in the specific activity was found when loaded or unloaded frog recti (briefly labelled with-orthophosphate) were contracted by treatment with KCl. Even in the absence of shortening (under isometric conditions or in the absence of calcium ions) a decrease in the specific activity occurred after depolarisation of the membranes. A similar change in specific activity was found when P-labelled glycerol-extracted psoas fibrils were treated with ATP or ITP in the presence of magnesium ions. Ho change occurred when the fibrils were treated with ATP and calcium ions. 2,4-Dinitrophenol did not inhibit the effect of ATP and magnesium ions on the bound phosphate of the myofibrils. In the intact muscle the ATP-p and the P of PC do not become30equally labelled when incubated with P-orthophosphate for 60 min. That material which is extracted as Pi by trichloracetic acid from acetone-dried washed muscle residues has a similar degree of labelling as the ATP-p is isolated from whole muscles.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Physiology