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Title: Investigation of the thermoinstability of cystic fibrosis mutants
Author: Wang, Yiting
ISNI:       0000 0004 6057 8656
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2015
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Cystic fibrosis (CF) is caused by dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. The most common CF mutation F508del produces a misfolded protein with three major defects: reduced protein processing; aberrant channel gating and thermo-instability. Low-temperature incubation partially rescues the plasma membrane expression of FS08del-CFTR. However, the channel is unstable and runs down within minutes when studied at 37°C in excised inside-out membrane patches. The aims of my research were to study wild-type and FS08del-CFTR channel behaviour at different temperatures and investigate thermoinstability of F508del-CFTR at the plasma membrane and effects of small-molecules.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available