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Title: Leveraging the power of mass spectrometry-based proteomics to reveal novel biological insights
Author: Dowle, Adam Ashley
ISNI:       0000 0004 5991 7788
Awarding Body: University of York
Current Institution: University of York
Date of Award: 2016
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Mass spectrometry-based proteomics has the potential to offer new qualitative and quantitative insights into a wide array of biological questions. The work presented in this thesis, including the associated submitted papers, evidences my contribution to this field. Paper 1 demonstrates the successful identification of post translational modifications imparted upon histones following DNA damage. Papers 2-4 employ a label-free quantification approach to study the immunomodulation molecules endemic parasites use to impair host resistance. Paper 5 uses an isobaric tagging approach to quantify changes in immune response when dendritic cells are exposed to medically important antigens. Paper 6 displays the power of mass spectrometry in identifying a novel co-factor, which could not be identified by X-ray crystallography. In paper 7 mass spectrometry is used to show that the remaining unidentified enzyme in the morphinan biosynthetic pathway is expressed as a fusion protein composed of two distinct enzymes. These studies are all linked in their reliance on my use of mass spectrometry-based proteomics to make original contributions to knowledge and understanding within their fields.
Supervisor: Thomas-Oates, Jane Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available