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Title: Analysis of Enterococcus faecalis mutants affected in septum cleavage during cell division
Author: Elsarmane, Fathe
ISNI:       0000 0004 5989 3587
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2016
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Enterococcus faecalis is a Gram-positive commensal bacterium forming diplococci/short chains that can also be an opportunistic pathogen. Some infections caused by E. faecalis such as endocarditis and bacteraemia can be life-threatening and difficult to treat due the resistance of this organism to several classes of antibiotics. Recent data revealed that the morphology of E. faecalis is an important factor for pathogenesis. Whilst strains forming diplococci or short chains can evade phagocytes and cause lethality in the zebrafish model of infection, isogenic derivatives forming long cell chains are more susceptible to phagocyte uptake and become avirulent. The enzyme dedicated to septum cleavage in E. faecalis, N-acetylglucosaminidase AtlA therefore has a key role during pathogenesis. The aim of this project was to identify genes involved in the control of AtlA activity during cell division. We used two distinct phenotypic screens to isolate spontaneous and transposon mutants impaired in septum cleavage. Our results indicate that several mechanisms ensure spatial and temporal control of AtlA enzymatic activity during growth.
Supervisor: Mesnage, Stephane Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available