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Title: Studies on Aspergillus fumigatus bronchopulmonary aspergillosis
Author: Morrison-Cleator, Sheila Fletcher
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1970
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In 262 chest patients at the City Hospital, Edinburgh, sputum samples were examined microscopically for aspergillus-type mycelium and sputum eosinophilia and cultured for aspergillus. Prick-skin and intradermal hypersensitivity tests to A.fumigatus antigens were carried out and the production of immediate and late reactions noted. A serum precipitin test to A.fumigatus antigens was performed, and precipitation reactions were observed after staining the medium in the agar gel double-diffusion test. Swabs from the upper respiratory tract were cultured for aspergillus. Of the 262 patients 31 were selected as regards a probable diagnosis of bronchopulmonary aspergillosis and 231 were unselected. Two types of growth of A.fumigatus in sputum cultures was observed: abundant growths considered significant and light growths not so considered. Contamination of culture plates with atmospheric A.fumigatus spores was a problem. There was a seasonal variation, with a winter peak, in the incidence of light growths of A.fumigatus from cultures of sputum and swabs and in the incidence of contaminant growths on culture plates. There was no association between isolation of A.fumigatus from swab cultures and abundant growths of A.fumigatus from sputum. Among unselected cases the incidence of significant growth of A.fumigatus from sputum was 10.8% and of other species of aspergillus 2.6%. The incidence of serum precipitating antibodies to A.fumigatus antigens was 3.4% and of positive reactions in skin hypersensitivity tests to A.fumigatus antigens 16.5%. Significant growth of A.fumigatus from sputum was associated with purulence of sputum and sputum eosinophilia. The intradermal test was considered superior to the prick-skin test in terms of the number of positive reactions obtained. The serum precipitin test appeared as the most reliable diagnostic index of bronchopulmonary aspergillosis. 22 cases were considered to have bronchopulmonary aspergillosis and factors in their environment and symptomatology are discussed. The incidence of known bronchopulmonary aspergillosis among chest patients at the hospital was 1.0%, and among unselected cases intensively studied 3.9%. A method for obtaining constant end-points in assessing the minimal inhibitory concentration of pimaricin for A.fumigatus strains is described.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available