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Title: The role of Epicatechin and Lycopene on the antiatherogenic properties of HDL
Author: Henry, Sarah-Louise
ISNI:       0000 0004 5988 9318
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2015
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Introduction: High Density Lipoproteins (HDL) possesses an array of atherogenic functions, which lower cardiovascular disease (CVD)-risk. However, HDL can become dysfunctional under inflammatory conditions, with proatherogenic potential. Research highlights the benefits of dietary interventions with fruit and vegetables to lower CVD-risk; however, research is limited as to their effect on HDL functionality. Aim: To investigate if specific fruits and, therefore, specific antioxidants, influenced nutritional status, biomarkers of CVD-risk and HDL functionality. The APPS Study investigated the role of epicatechin derived from apples/apple products and the lycopene Study investigated the role,of lycopene derived from tomatoes/lycopene supplement. Additionally, we investigated if epicatechin and Iycopene influenced HDL functionality in vitro. Methods: Nutritional status was assessed by food diaries/serum analysis. HDL subfractions were isolated from serum by rapid ultracentrifugation. HDL functionality was assessed by measuring SAA (ELlSA), protein and apoAI concentration (spectrophotometric and SRID, respectively), and PON-1, CETP and LCAT activities (spectrophotometric and f1uorimetric assays). In vitro, HDL was incubated with increasing concentrations of epicatechin or lycopene and changes to HDL function was assessed. Results: The APPS Study identified that epicatechin did not influence CVD-risk, but had the potential to increase HDL3-LCAT activity. The Lycopene Study identified that serum Iycopene was associated with change in IL-6 and SAA. In addition, increased Iycopene intake negated SAA's association with HDL3 and augmented the activities of HDL3-PON-1 and HDL3-LCAT, as well as reducing HDL2- and HDL3-CETP activity. In vitro, epicatechin enhanced HDL3-PON-1 activity and decreased HDL3-CETP activity at higher concentrations, and lycopene enhanced HDL3-PON-1 activity at higher concentrations. Conclusion: We have demonstrated that consumption of fruits, and their specific anti-oxidants, epicatechin and lycopene, were capable of interacting with, and influencing HDL's functional aspects, which may augment the anti-atherogenic functions of this lipoprotein and have the potential to reduce CVD-risk.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available