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Title: Investigations on the serotypes and virulence profiles of non-O157 Shiga-toxin producing Escherichia coli (STEC) and Enteropathogenic Escherichia coli (EPEC) isolated from bovine farms and abattoirs
Author: Monaghan, Áine Marie
Awarding Body: Ulster University
Current Institution: Ulster University
Date of Award: 2012
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This study focuses on emerging E. coil serotypes and has developed methods for the isolation and identification of non-0157 STEC and EPEC. A basal medium for the isolation of these pathogens was developed as well as a serogroup specific PCR assay for the detection of the 02 serogroup. These culture and molecular based techniques have proven to be valuable in the detection, identification, and epidemiological investigation of these groups of emerging pathogens. These methods were applied to 1) a farm study, whereby samples (faecal and soil) and 2) an abattoir study, whereby samples (hide and carcass) were analysed for the presence of non-0157 STEC and EPEC. Isolates were subsequently characterised in terms of serotype/serogroup and virulence markers. The data generated by this work has illustrated the extent of non-0157 STEC and EPEC contamination in the farm and abattoir environments, thus providing scientific background upon which control strategies may be based.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Non-0157 Escherichia coli, STEC, emerging pathogens, farm survey, abattoir study, Escherichia coli 0113, faecal samples, soil samples, hide samples, carcass swabs, survival of non-0157 STEC