Mycotoxins, heavy metals and pesticides are ubiquitous in the environment, contributing to multiple contamination of foodstuff. However, due to complications of determining their combined toxicity, legal limits of exposure are determined for single compounds, based on long standing toxicological techniques. Governmental regulatory bodies must begin to contemplate how to safeguard the population when such mixtures of contaminants are present in food. The aim of this thesis was to study the interactive and cytotoxic effects .of mixtures of chemical food contaminants when present at their corresponding regulatory limits, the capability of high content analysis (HCA)at determining these biological effects and to determine possible mechanistic approaches behind the adverse effects measured.