This work contributes to the development of electrocatalysts for use in polymer electrolyte fuel cells, specifically for the cathodic oxygen reduction reaction (ORR). To achieve this, electrochemical analysis was conducted using biofabricated platinum (bio-Pt) catalyst. Bio-Pt per se was found to be a poor catalyst for the ORR, attributed to the platinum being inaccessible to the reactants. Various ‘cleaning’ techniques were tested to partially remove biomass, providing improved catalytic activity.