The demand for nurse entrepreneurial education has steadily increased around the globe in order to meet the changing health care needs of the public. Many universities in countries such as USA and Australia have already launched a wide range of nursing entrepreneurial programmes. Although Thai nursing education has also realised the important role of nurse entrepreneurs to Thai healthcare modernisation, there are no training courses which are designed to equip nurses with entrepreneurial skills. This action research aimed to study Nursing and Business faculty collaboration established through an action research project in order to develop a nurse entrepreneur training programme and to evaluate the effectiveness of the collaboration team at Assumption University in Thailand. This collaboration team (Ad-hoc Module Development Committee) consisted of twelve members: three nursing faculty, three business faculty, three business entrepreneurs and three nursing entrepreneurs. The model of team effectiveness, developed by West et al. (1998), was used to analyse qualitative and quantitative data in order to evaluate team performance of the AMDC. The results demonstrated important characteristics in terms of input and process factors, which greatly contributed to team effectiveness and successful outcomes of the AMDC. Three input characteristics enhancing the effective collaboration were identified: intrinsic task motivation, the appropriate mix of cross-faculty team, and the collective culture of the team. Three processes, which were shown to be significant for delivering high team performance of the AMDC, were positive participatory climate, effective shared leadership and skilful communication of the team. These input and process characteristics provided a strong foundation for the AMDC to achieve high performance outcomes with greater benefits of the programme product than envisioned in the original goal. However, committee selection process, high workload demands of members, and limited minority influence were identified as potential issues, which could hinder the development of interdisciplinary course.