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Title: The evolution and transmission of HA-MRSA ST239 through hospitals in Turkey and intercontinental spread
Author: Aldeljawi, Mona
ISNI:       0000 0004 5371 7128
Awarding Body: University of Bath
Current Institution: University of Bath
Date of Award: 2015
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Next-generation sequencing technology provides high-resolution data for epidemiological surveillance of bacterial pathogens on local and global scales. This approach has been used for many species including Methicillin Resistant Staphylococcus aureus (MRSA). In this thesis I demonstrate the utility of these data for understanding the spread of the globally disseminated clone MRSA ST239. I focus both on local and national-level epidemiology through sequence data of 71 isolates recovered from four hospitals representing three cities in Turkey; Istanbul (x2). Ankara and Izmir. I analyse whole genome sequence data from a further 33 ST239 isolates from global sources. These data were combined with previously published data for phylogenetic analysis based only on the core genome. I demonstrate how transmission events can be inferred from this approach on multiple levels; within hospital, between hospitals and between countries. The data pointed to a European origin of ST239, and independent introductions from Europe to Turkey, South America and East Asia. I also demonstrate how whole genome sequence data can be used to develop bespoke PCR assays, based on phage variation, for rapid local epidemiology. Finally, I consider how the sequence data might be used to explain variation in virulence potential, and describe the distribution and transfer of an important phage-borne virulence determinant, sasX, within Europe. Finally, I identified a single isolate with very strong biofilm forming ability likely due to the over-expression of the important adhesion SasG.
Supervisor: Feil, Edward ; Massey, Ruth Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: MRSA ; ST239 ; Whole Genome Sequencing ; SasG ; Next Generation Sequencing ; HA-MRSA (ST 239) ; NGS ; Dokuz Eylu¨l hospital ; intercontinental transmission ; Hacettepe hospital ; Cerrahpasa hospital ; Marmara hospital ; Turkey