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Title: The modification of biotin analogues to function as multipurpose labelling tools
Author: Sehmi, Inderpal Kaur
ISNI:       0000 0004 5362 6285
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2014
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This project has focused on establishing a specific multipurpose labelling system via the modification of D-( + )-biotin in use with (strept)avidin proteins for purification and isolation. Two biotin analogues; 2-azidobiotin and 2-propargylbiotin, have been synthesised, introducing an alkyne and azide group at the 2 position of the valeryl side chain of biotin respectively. Isothermal titration calorimetry studies showed that the biotin analogues had a reduced affinity (Kd= 1V10-9 M) for strept(avidin) proteins, when compared to biotin (Kd= ~10-15 M).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available