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Title: Telomeres and related repetitive DNA in the mouse genome
Author: Starling, Jacqueline
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1992
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This project was designed to isolate and characterise interstitial telomere repeat containing loci from the human and mouse genomes and to investigate the nature of the mouse telomere. Cloning of the internal telomere repeat loci proved to be extremely difficult and so alternative methods such as restriction enzyme analysis, hybridisation analysis, inheritance studies, and mapping within recombinant inbred and backcross mouse strains were employed to characterise these regions within the mouse genome. Similar methods were used to characterise mouse telomeres. From these experiments it was shown that, in the mouse, Trypanosoma-like (TTAGGG)n telomere repeats are present at the telomeres and at interstitial sites. Within both of these regions, the (TTAGGG)n repeats are present within distinct genetic loci that are stably inherited through subsequent generations. New variant generation is observed at both types of loci, takes place at a significantly higher frequency at the telomeric compared to interstitial loci and occurs during gametogenesis. It is possible that the higher rate of new variant generation at mouse telomers compared to internal sites may relate to their position within the mouse genome. Restriction enzyme and hybridisation sequence analysis demonstrated that both classes of loci are composed of telomere-related repeats and that an undefined simple repeat may also be present. Direct sequencing is required before the nature and organisation of simple repetitive DNA within these loci can be determined. Mapping of the interstitial, (TTAGGG)n telomere repeat containing loci within the BxD Rl and Mus spretus/C57Bl/6 backcross mice demonstrated their presence within the protermini of chromosomes 9, 13 and X. It remains to be determined whether this distribution is functionally significant.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available