Use this URL to cite or link to this record in EThOS:
Title: Studies of paratuberculosis of red deer
Author: Nyange, John Frederick Charles
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1991
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
The mesenteric lymph nodes of 167 apparently healthy slaughtered red deer (18-24 m.o. old) from a herd in which several cases of paratuberculosis had occurred were examined by histopathology. An infection rate of 38% was found suggesting a high susceptibility of deer to paratuberculosis. Vaccination of all day-old calves with a standard bovine paratuberculosis vaccine stopped the occurrence of disease in yearlings and reduced significantly the rate (64/167 vs 32/201 p < < 0.001) and severity (41/64 vs 4/32 p < < 0.001) of infection as assessed by lymph node pathology of normal slaughter deer. The pathological lesions of paratuberculosis as observed in naturally and experimentally infected red deer, were inconsistent. Nevertheless, the specific lesions were basically similar to those of the disease in other ruminants, especially sheep and goats, where in severe cases, focal necrosis, mineralization and reactive fibrosis accompanied the granulomatous lesions. The clinical and pathological findings of avian tuberculosis in red deer, in a single natural case and in experimentally infected animals, were similar to those of paratuberculosis but they were more severe and widely disseminated into organs other than the intestines and related lymph nodes. The pathogeneses of paratuberculosis and avian tuberculosis in very young deer and very young sheep were shown to be similar by experimental infection. However the two infections progressed more rapidly and severely in deer than in sheep, with avian tuberculosis being more acute and severe than paratuberculosis. Fifty-four isolates of M. paratuberculosis from red deer were shown by cultural characterization to be similar to one another and to isolates of the organisms from a cow and a goat. Analysis of 14 of the isolates by SDS-PAGE and Western blotting did not distinguish them from one another or the bovine and caprine isolates. Despite many similarities in their polypeptide patterns, they were shown to differ from an atypical isolate of mycobacterium (M928), a commercial protoplasmic antigen (PPA-3) and M. avium isolates. Further analysis of six of the isolates by genomic DNA RE digestion with BamHI, PstI and EcoRI and hybridization with 32P-PCR279 (a DNA probe specific for M. ptbc), showed them to be homologous to both the bovine and caprine isolates when using BamHI and PstI, and only to the caprine isolate when using EcoRI. In conclusion, paratuberculosis of deer is basically similar to that of other ruminants, especially sheep and goats. However the short course and severity of disease in young animals is novel, contrasting with cattle, sheep and goats in which older animals become clinically affected.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available