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Title: The control of arachidonic acid turnover in relation to prostaglandin production by the guinea-pig uterus
Author: Norman, Sophie Johanna
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1998
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ACS, ACLS and PLA2 activities were detected in guinea-pig endometrium on both 7 (day of low PGF output) and day 15 (day of high PGF output) of the oestrous cycle and in the endometrium of ovariectomizd guinea-pigs treated with oestradiol and/or progesterone. Treatment with aristolochic acid (ARA) and quinacrine (QUIN) (PLA2 inhibitors) significantly (P ¸0.05) reduced PG output from both day 7 and day 15 endometrium cultured for 24 h, demonstrating the crucial role that PLA2 plays in the regulation of AA release for PGF synthesis in the guinea-pig. Unexpectedly, treatment with p-hydroxymercuribenzoic acid (HMB0 and thimerosal (THM) (ACS and ACLS inhibitors) also significantly (P < 0.05) decreased PGF output. However, during long term inhibition of ACS and ACLS, since the rate of uptake of AA into lysophospholipids will be reduced, the amount of AA appropriately placed in the sn-2 position of appropriate phospholipids for the action of PLA2 will also be reduced. Therefore, PLA2 may be indirectly inhibited by a lack of substrate. ACS, ACLS and PLA2 activities were detected in the endometrium and conceptus of early pregnant (day 15) guinea-pigs. All three enzymes were also detected in the endometrium, chorio-allantoic placenta, chorion and amnion of day 29 and 36 pregnant guinea-pigs. Treatment with THM and ARA of day 22, 29 and 36 pregnant guinea-pig endometrial and fetal tissues during 24 h culture suggested that the control of AA uptake is important in the maternal placenta, fetal placenta, chorion and amnion, and that PLA2 appears to have an essential role in the control of PG synthesis from the endometrium, chorion and amnion of pregnant guinea-pigs. ACS, ACLS and PLA2 may have a role in the control of arachidonic acid turnover, and therefore PG production, in guinea-pig uterine and fetal tissues. The stimulus responsible for increased PLA2 activity towards the end of the cycle, and the mechanism of the anti-luteolytic factor provided by the guinea-pig conceptus, remains obscure. The control of AA uptake in the placenta and fetal membranes seems to have a role in the delicate regulation of PG synthesis during pregnancy.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available