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Title: Mesenteric fat in Crohn's disease
Author: Broadhurst, J. F.
ISNI:       0000 0004 5359 4527
Awarding Body: University College London (University of London)
Current Institution: University College London (University of London)
Date of Award: 2015
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Crohn’s Disease is a chronic inflammatory disorder of the bowel affecting approximately 1 in 800 people in the UK. The terminal ileum is most commonly affected and the mesentery becomes thickened, a phenomenon known as ‘fat wrapping’. The cause is not understood. Elemental feeding can induce remission in Crohn’s disease and polyunsaturated fatty acid (PUFA) content may be the cause of a reduction in inflammation. Particular attention has focused on n-­‐3 and n-­‐6 PUFA content of elemental feeds. The aim of this study was to further characterize mesenteric fat in Crohn’s disease and to examine the effects of different PUFA on mesenteric inflammation in vitro. Samples of adipose tissue were collected from patients undergoing intestinal resection for Crohn’s disease and from controls. These were cultured in media and elemental (E028 and Emsogen) feeds containing different concentrations of n-­‐3 and n-­‐6 PUFA. Significant findings were that mesenteric (MF) and omental (OM) adipose tissue released more inflammatory cytokines IL-­‐6, leptin and MCP-­‐1 when cultured in media rich in n-­‐6 PUFA compared to media rich in n-­‐3 PUFAs. OM mean IL-­‐6 concentrations were 18.6(3.1-­‐21.8)ng/mL in n-­‐6 PUFA vs 3.07(0.62-­‐19.10)ng/mL in n-­‐3 PUFA (p=0.018), MF IL-­‐6 concentrations were 3.77(0.76-­‐9.52)ng/mL in n-­‐6 PUFA vs 1.5(0.42-­‐2.61)ng/mL in n-­‐3 PUFA (p=0.03). OM Leptin concentrations were 0.42(0.08-­‐0.90)ng/mL in n-­‐6 PUFA vs 0.08(0.07-­‐0.14)ng/mL in n-­‐3 PUFA (p=0.006), MF Leptin concentrations were 0.27(0.13-­‐2.62)ng/mL in n-­‐6 PUFA vs 0.12(0.07-­‐0.31)ng/mL in n-­‐3 PUFA (p=0.033). OM MCP-­‐1 concentrations were 18.80(4.39-­‐31.5)ng/mL in n-­‐6 PUFA vs 1.83(0.69-­‐4.82)ng/mL in n-­‐3 PUFA (p=0.002) and MF MCP-­‐1 concentration were 4.59(2.20-­‐13.72)ng/mL in n-­‐6 PUFA vs 1.20(0.82-­‐3.39)ng/mL in n-­‐3 PUFA (p=0.006). These findings show that n-­‐6 PUFAs stimulate a greater inflammatory response from omental and mesenteric fat in vitro and may assist in formulating a more effective elemental feed for inducing remission in patients with active flares of Crohn’s disease.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available