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Title: The identification and validation of drug targets for prostate cancer using integrated approaches
Author: Oloko, Olayinka Azeez
ISNI:       0000 0004 5358 6092
Awarding Body: University of Essex
Current Institution: University of Essex
Date of Award: 2014
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Prostate cancer is a very complex disease, which we still do not fully understand. It is the second most common cause of cancer- related death in men and therefore, it is very important to investigate the mechanisms of prostate tumorigenesis and drug development. Membrane proteins represent a valuable source of potential drug targets due to their intimate involvement in a wide variety of disease states including but not limited to cancer. In our study, we identified five drug targets, namely; EpCAM, TACSTD2, FAM3C, NCEHl and TMEM109 abundantly present in the membrane fraction of prostate cancer cell lines using proteomic approaches and validation of these proteins by other biological techniques. The cumulative data from our study indicate that global levels of these five candidates either at mRNA or protein levels are more present in prostate cancer cell lines and tissues than their normal counterparts. We also found by ICC/IHC/IF that these five drug targets are subcellularly localized in the cytoplasmic membrane region of prostate cancer cells and on the basolateral surfaces of prostate tumour tissues. Taking into account the link between T ACSTD2 and F AM3C to clinicopathological data of prostate tumour tissues stained and the MTT survival assay carried out on prostate cancer cell lines, our data suggest that overexpression ofTACSTD2 and FAM3C leads to poor prognosis in prostate cancer patients. We also tried to study the relationship between our drug targets, CTCF and BORIS. Our preliminary data shows a significant effect of CTCF and none of BORIS on our drug targets in only DU145 cell lines. Suggesting these to be cell line specific and CTCF may play a role in the transcriptional regulation of our target genes. Although, recently some of these proteins have been widely explored, their function and the role they play in cancer development still remain elusive.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available