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Title: Cell fate in the developing mouse neocortex
Author: Levers, Teresa Elizabeth
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2000
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During neocortical development the timing of proliferation, migration and differentiation of neurogenesis is well understood. Gliogenesis (with the exception of radial glia) is known to take place after neurogenesis and continue into the postnatal period. However, the exact timings of proliferation, migration and differentiation of glial cells remains largely unknown. To further investigate the timings of gliogenesis in the mouse neocortex, 5-bromo-2-deoxyuridine (BrdU) was used to label proliferating cells in vivo around the time that neurogenesis was ending. The proliferative and migratory behaviour of these cells was followed as they populated the cortical wall. Furthermore, immunocytochemical markers of neurones and glia were used to examine the fates of these cells. The exact time point at which neurogenesis finished and gliogenesis begins within the mouse neocortex was established. Secondly, a study of the mechanisms involved in neuronal and glial cell fate decisions was investigated. For example, how does a progenitor cell know to become a neuron or a glial cell? A tissue culture technique was devised to study the possible roles of environmental factors versus cell autonomous mechanisms in cell fate decisions. The effects of intrinsic versus extrinsic factors were examined further using a heterochronic in vivo transplant technique. Results from the above two approaches suggest that the environment does play a role in cell fate decisions within the mouse neocortex. Finally, a subtractive cDNA library was screened to isolate and identify rare and novel genes, which are likely to be associated with neuronal/glial cell fate decisions. A novel RNA binding protein was isolated and its expression pattern was characterized in the developing mouse brain.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available